What question did this study set out to answer?

Investigate how Parkin coordinates mitofusin degradation and mitophagy to selectively eliminate damaged mitochondria.

December 20, 2010The Journal of Cell BiologyOpen Access

Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin

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Authors

ATAtsushi TanakaHyogo Prefectural Amagasaki General Medical Center MCMegan M. ClelandMedical College of Wisconsin SXShan XuFirst Affiliated Hospital of Xi'an Jiaotong University

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Implication

Experimental study reveals that proteasome and p97 drive Parkin-dependent mitofusin degradation in depolarized cells, indicating a mechanism preventing damaged mitochondrial refusion.

Key Points

Investigate how Parkin coordinates mitofusin degradation and mitophagy to selectively eliminate damaged mitochondria.
Evaluated Parkin-mediated ubiquitination and degradation of mitofusins Mfn1 and Mfn2 in depolarized cell models.
Tested the functional necessity of the AAA+ ATPase p97, proteasomal activity, and Drp1-driven fission in mitochondrial clearance.
Parkin induces ubiquitination and subsequent degradation of Mfn1 and Mfn2 through a proteasome- and p97-dependent pathway upon mitochondrial depolarization.
Depolarization prompts p97 recruitment to mitochondria, where it degrades mitofusins to prevent or delay refusion of impaired mitochondria.
Pharmacological or genetic inhibition of the proteasome, p97, or Drp1-mediated mitochondrial fission completely blocks Parkin-induced mitophagy.

Cite This Study

Tanaka et al. (2010) studied this question.

synapsesocial.com/papers/69db0d830d8d6ef495a3cb25 https://doi.org/10.1083/jcb.201007013

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Implication

Experimental study reveals that proteasome and p97 drive Parkin-dependent mitofusin degradation in depolarized cells, indicating a mechanism preventing damaged mitochondrial refusion.

Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin

Discussion

Member takes

Implication

Key Points

Cite This Study

Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin

Discussion

Member takes

Implication

Key Points

Cite This Study

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