A rapid, sensitive, and convenient spectrophotometric assay was developed and characterized for measurement of proteolysis of milk proteins in buffered solutions or in milk. a-Amino groups released by hydrolysis react with o-phthaldialdehyde and /3-mercaptoethanol to form an adduct that absorbs strongly at 340 nm. The absorptivity (e = 6000 M -1 cm -1) is similar for all oe-amino groups.
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Church et al. (1983) studied this question.