What question did this study set out to answer?

To identify the specific E3 protein ligase that coordinates type I interferon-induced ISG15 conjugation to cellular protein targets.

July 1, 2006Proceedings of the National Academy of SciencesOpen Access

HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets

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Authors

JWJoyce Jing Yi Wong YPYuh Fen PungUniversity of Nottingham Malaysia Campus SSSiu Kwan SzeBrock University

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Implication

Experimental study reveals that HERC5 mediates type I interferon-induced ISG15 conjugation in cellular targets, indicating an essential catalytic role for innate immune signaling.

Key Points

To identify the specific E3 protein ligase that coordinates type I interferon-induced ISG15 conjugation to cellular protein targets.
Assessed ISG15 target modification after interferon induction and under HERC5 overexpression conditions.
Used small interfering RNA (siRNA) knockdown to silence endogenous HERC5 and observe the effect on interferon-induced ISGylation.
Reconstituted ISG15 conjugation by coexpressing HERC5, Ube1L, and UbcH8 in vivo, and engineered a Cys-994 to Ala mutation within the HERC5 HECT domain.
Silencing endogenous HERC5 via siRNA completely blocked interferon-induced ISG15 conjugation to target proteins.
Coexpression of HERC5 with Ube1L and UbcH8 successfully triggered ISG15 conjugation in vivo without requiring interferon stimulation.
Substitution of Cys-994 to Ala in the HECT domain completely abolished the E3 protein ligase activity of HERC5.

Cite This Study

Wong et al. (2006) studied this question.

synapsesocial.com/papers/69db1d7d0d8d6ef495a3cde5 https://doi.org/10.1073/pnas.0600397103

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Implication

Experimental study reveals that HERC5 mediates type I interferon-induced ISG15 conjugation in cellular targets, indicating an essential catalytic role for innate immune signaling.

HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets

Discussion

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Implication

Key Points

Cite This Study

HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets

Discussion

Member takes

Implication

Key Points

Cite This Study

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