What question did this study set out to answer?

To determine how the novel chromosome-associated protein LAB-1 protects meiotic sister chromatid cohesion and regulates the Aurora B kinase AIR-2 in holocentric Caenorhabditis elegans.

October 15, 2008Genes & DevelopmentOpen Access

LAB-1 antagonizes the Aurora B kinase in C. elegans

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Authors

CCCarlos Egydio de CarvalhoUniversity of Saskatchewan SZSophie ZaaijerUniversity of California, Riverside SSSarit SmolikovHarvard University

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Implication

Genetic analysis reveals LAB-1 antagonizes Aurora B kinase AIR-2 to protect cohesin in Caenorhabditis elegans, indicating a shugoshin-independent chromosome segregation mechanism.

Key Points

To determine how the novel chromosome-associated protein LAB-1 protects meiotic sister chromatid cohesion and regulates the Aurora B kinase AIR-2 in holocentric Caenorhabditis elegans.
Assessed chromosome segregation, cohesin dynamics, and protein interactions in wild-type, mutant, and RNAi-knockdown Caenorhabditis elegans strains.
Tracked the subcellular localization of LAB-1, AIR-2, and Shugoshin in meiotic germ cells and dividing embryos using GFP fusion constructs and immunofluorescence.
LAB-1 preserved meiotic sister chromatid cohesion by confining the Aurora B kinase AIR-2 to the homolog interface via the PP1/Glc7 phosphatase GSP-2.
Depleting LAB-1 suppressed mitotic division defects in air-2 mutant embryos, indicating that LAB-1 globally antagonizes AIR-2 across both meiosis and mitosis.
Caenorhabditis elegans Shugoshin was dispensable for cohesin protection but was required to maintain chromosome segregation fidelity at metaphase I.

Cite This Study

Carvalho et al. (2008) studied this question.

synapsesocial.com/papers/69dba40750e1971baba3c19a https://doi.org/10.1101/gad.1691208

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Implication

Genetic analysis reveals LAB-1 antagonizes Aurora B kinase AIR-2 to protect cohesin in Caenorhabditis elegans, indicating a shugoshin-independent chromosome segregation mechanism.

LAB-1 antagonizes the Aurora B kinase in C. elegans

Discussion

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Implication

Key Points

Cite This Study

LAB-1 antagonizes the Aurora B kinase in C. elegans

Discussion

Member takes

Implication

Key Points

Cite This Study

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