What question did this study set out to answer?

Determine whether the Caenorhabditis elegans Aurora B kinase AIR-2 regulates the meiotic and mitotic spindle localization of the BimC kinesin BMK-1.

November 17, 2004Molecular Biology of the CellOpen Access

The Caenorhabditis elegans Aurora B Kinase AIR-2 Phosphorylates and Is Required for the Localization of a BimC Kinesin to Meiotic and Mitotic Spindles

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Authors

JBJohn D. BishopThe University of Texas MD Anderson Cancer Center ZHZhenbo HanIllinois College JSJill M. SchumacherThe University of Texas MD Anderson Cancer Center

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Implication

Laboratory study demonstrates that AIR-2 phosphorylates BMK-1 to direct its spindle localization in C. elegans embryos, indicating a recruitment role for Aurora B kinases.

Key Points

Determine whether the Caenorhabditis elegans Aurora B kinase AIR-2 regulates the meiotic and mitotic spindle localization of the BimC kinesin BMK-1.
Analyzed the spatial and temporal localization of BMK-1 in wild-type, AIR-2-deficient, and ZEN-4-deficient C. elegans embryos.
Tested direct protein-protein interactions and in vitro phosphorylation between AIR-2 and the BMK-1 C-terminal tail domain.
BMK-1 localizes to meiotic spindles, mitotic spindle microtubules from prophase to anaphase, and the spindle midzone during anaphase and telophase in wild-type embryos.
Depletion of AIR-2 greatly reduces BMK-1 spindle localization, an effect not observed in ZEN-4-deficient embryos.
AIR-2 directly interacts with BMK-1 and specifically phosphorylates its C-terminal tail domain in vitro.

Cite This Study

Bishop et al. (2004) studied this question.

synapsesocial.com/papers/6a89ca5a5c716ca1a5d45804 https://doi.org/10.1091/mbc.e04-08-0682

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Implication

Laboratory study demonstrates that AIR-2 phosphorylates BMK-1 to direct its spindle localization in C. elegans embryos, indicating a recruitment role for Aurora B kinases.

The Caenorhabditis elegans Aurora B Kinase AIR-2 Phosphorylates and Is Required for the Localization of a BimC Kinesin to Meiotic and Mitotic Spindles

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Cite This Study

The Caenorhabditis elegans Aurora B Kinase AIR-2 Phosphorylates and Is Required for the Localization of a BimC Kinesin to Meiotic and Mitotic Spindles

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Cite This Study

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