What question did this study set out to answer?

To determine the molecular mechanisms through which G protein-coupled receptor activation triggers receptor tyrosine kinase transactivation and downstream extracellular signal-regulated kinase signaling.

March 1, 2000Journal of Biological ChemistryOpen Access

The β2-Adrenergic Receptor Mediates Extracellular Signal-regulated Kinase Activation via Assembly of a Multi-receptor Complex with the Epidermal Growth Factor Receptor

View Full Paper

Ask AI

Bookmark

Authors

SMStuart MaudsleyMoffitt Cancer Center KPKristen L. PierceHoward Hughes Medical Institute AZA.M. ZamahUniversity of Chicago

Discussion

Member takes

Implication

Laboratory study demonstrates beta-2 adrenergic receptors activate ERK via EGFR transactivation in COS-7 cells, indicating receptor cross-talk requires multireceptor assembly and endocytosis.

Key Points

To determine the molecular mechanisms through which G protein-coupled receptor activation triggers receptor tyrosine kinase transactivation and downstream extracellular signal-regulated kinase signaling.
Stimulated COS-7 cells expressing beta-2 adrenergic receptors with the agonist isoproterenol to evaluate epidermal growth factor receptor responses.
Assessed receptor dimerization, internalization, physical complex assembly, and ERK1/2 phosphorylation in the presence of selective inhibitors of EGFR, Src kinase, and clathrin-mediated endocytosis.
Beta-2 adrenergic receptor stimulation triggered epidermal growth factor receptor dimerization, autophosphorylation, internalization, and the formation of a physical multireceptor complex containing both receptors.
Activation of ERK1/2 downstream of the beta-2 adrenergic receptor was suppressed by pharmacological inhibition of EGFR, Src kinases, or clathrin-mediated endocytic machinery.

Cite This Study

Maudsley et al. (2000) studied this question.

synapsesocial.com/papers/69dbc7d97d378569a9835bfa https://doi.org/10.1074/jbc.275.13.9572

View Full Paper

Ask AI

Bookmark

Implication

Laboratory study demonstrates beta-2 adrenergic receptors activate ERK via EGFR transactivation in COS-7 cells, indicating receptor cross-talk requires multireceptor assembly and endocytosis.

The β2-Adrenergic Receptor Mediates Extracellular Signal-regulated Kinase Activation via Assembly of a Multi-receptor Complex with the Epidermal Growth Factor Receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

The β2-Adrenergic Receptor Mediates Extracellular Signal-regulated Kinase Activation via Assembly of a Multi-receptor Complex with the Epidermal Growth Factor Receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider