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Develop and validate a reproducible method to isolate and cultivate separate, highly purified populations of astrocytes and oligodendrocytes from postnatal mammalian brain tissue.

June 1, 1980The Journal of Cell BiologyOpen Access

Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.

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Develop and validate a reproducible method to isolate and cultivate separate, highly purified populations of astrocytes and oligodendrocytes from postnatal mammalian brain tissue.
Cultured postnatal rat cerebral cells to exploit the natural stratification of oligodendrocytes growing atop an astroglial basal layer in the absence of viable neurons.
Applied mechanical shear forces using an orbital shaker for 15–18 hours at 37 °C to selectively detach the overlying oligodendrocytes.
Characterized purity and function using electron microscopy, biochemical assays for glycerol phosphate dehydrogenase and 2',3'-cyclic nucleotide 3'-phosphohydrolase, and cyclic AMP responsiveness.
Yielded separated astrocyte and oligodendrocyte cultures with greater than 98% purity, producing approximately 1–2 × 10^7 viable cells (20–40 mg total cell protein).
Observed induction of astroglial process outgrowth after treatment with brain extract and dibutyryl cyclic AMP, without inducing visible morphological shifts in oligodendrocytes.

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Implication

Laboratory study demonstrates high-purity separation of astrocytes and oligodendrocytes from rat cerebra, highlighting a scalable platform to study glial biochemistry and physiology.

Cite This Study

McCarthy et al. (1980) studied this question.

synapsesocial.com/papers/69dbe6039e6f14d6f1684cd3 https://doi.org/10.1083/jcb.85.3.890

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Implication

Laboratory study demonstrates high-purity separation of astrocytes and oligodendrocytes from rat cerebra, highlighting a scalable platform to study glial biochemistry and physiology.

Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.

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Discussion

Implication

Cite This Study

Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.

Key Points

Discussion

Implication

Cite This Study

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