What question did this study set out to answer?

To evaluate whether the addition of trehalose protects pancreatic beta-cell exosome-like vesicles from aggregation and loss of bioactivity during isolation and repeated freeze-thaw cycles.

November 8, 2016Scientific ReportsOpen Access

Trehalose prevents aggregation of exosomes and cryodamage

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Authors

SBSteffi BöschInstitut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement LBLaurence de BeaurepaireInstitut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement MAMarie AllardAssistance Publique – Hôpitaux de Paris

Discussion

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Implication

Experimental study demonstrates trehalose prevents aggregation and cryodamage in pancreatic beta-cell exosomes, indicating improved preservation of vesicle bioactivity.

Key Points

To evaluate whether the addition of trehalose protects pancreatic beta-cell exosome-like vesicles from aggregation and loss of bioactivity during isolation and repeated freeze-thaw cycles.
Isolated pancreatic beta-cell exosome-like vesicles via differential centrifugation in phosphate-buffered saline (PBS) with or without 25 mM trehalose.
Assessed vesicle size distribution and concentration across repeated freeze-thaw cycles, and analyzed membrane integrity using cryo-electron tomography.
Measured preservation of vesicle biological activity via TNF-alpha cytokine secretion stimulation indexes in macrophage immune assays.
Supplementation with 25 mM trehalose narrowed particle size distribution and increased individual particle counts per microgram of protein.
Repeated freeze-thaw cycles increased particle concentration and distribution width in PBS-only controls, but not in samples containing 25 mM trehalose.
Beta-cell extracellular vesicles stored in trehalose showed consistently higher macrophage TNF-alpha stimulation indexes without visible lysis on cryo-electron tomography.

Cite This Study

Bösch et al. (2016) studied this question.

synapsesocial.com/papers/69dbed7940b636d1dda3c538 https://doi.org/10.1038/srep36162

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November 8, 2016Scientific ReportsOpen Access

Trehalose prevents aggregation of exosomes and cryodamage

View Full Paper

Ask AI

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Authors

Discussion

Member takes

Implication

Experimental study demonstrates trehalose prevents aggregation and cryodamage in pancreatic beta-cell exosomes, indicating improved preservation of vesicle bioactivity.

Key Points

To evaluate whether the addition of trehalose protects pancreatic beta-cell exosome-like vesicles from aggregation and loss of bioactivity during isolation and repeated freeze-thaw cycles.
Isolated pancreatic beta-cell exosome-like vesicles via differential centrifugation in phosphate-buffered saline (PBS) with or without 25 mM trehalose.
Assessed vesicle size distribution and concentration across repeated freeze-thaw cycles, and analyzed membrane integrity using cryo-electron tomography.
Measured preservation of vesicle biological activity via TNF-alpha cytokine secretion stimulation indexes in macrophage immune assays.
Supplementation with 25 mM trehalose narrowed particle size distribution and increased individual particle counts per microgram of protein.
Repeated freeze-thaw cycles increased particle concentration and distribution width in PBS-only controls, but not in samples containing 25 mM trehalose.
Beta-cell extracellular vesicles stored in trehalose showed consistently higher macrophage TNF-alpha stimulation indexes without visible lysis on cryo-electron tomography.

Cite This Study

Bösch et al. (2016) studied this question.

synapsesocial.com/papers/69dbed7940b636d1dda3c538 https://doi.org/10.1038/srep36162

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