What question did this study set out to answer?

This research aims to investigate the influence of polyribonucleotides on cell-free protein synthesis in E. coli.

October 1, 1961Proceedings of the National Academy of SciencesOpen Access

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

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Authors

MNMarshall W. NirenbergKanazawa University JMJohannes MatthaeiKlinikum Kassel

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Implication

Randomized trial shows the effect of polyribonucleotides on protein synthesis in E. coli, suggesting important implications for understanding protein production.

Key Points

This research aims to investigate the influence of polyribonucleotides on cell-free protein synthesis in E. coli.
Developed a cell-free E. coli extract for amino acid incorporation into proteins.
Extracts were dialyzed and stored at -150°C to maintain activity.
Assessed the effects of ribosomes and various inhibitors on amino acid incorporation.
Amino acid incorporation required ribosomes, ATP, and supernatant fractions.
Incorporation was stimulated by a mixture of L-amino acids and inhibited by puromycin and RNAase.
Initial incorporation rates were unaffected by DNAase, while later rates were significantly inhibited.

Cite This Study

Nirenberg et al. (1961) studied this question.

synapsesocial.com/papers/69dc1ba521d1c80c0f751cdb https://doi.org/10.1073/pnas.47.10.1588

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Implication

Randomized trial shows the effect of polyribonucleotides on protein synthesis in E. coli, suggesting important implications for understanding protein production.

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

Discussion

Member takes

Implication

Key Points

Cite This Study

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

Discussion

Member takes

Implication

Key Points

Cite This Study

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