What question did this study set out to answer?

To develop a cell-free system that allows for prolonged nuclear-directed transcription using nuclei and cytoplasm.

Prolonged Transcription in a Cell-Free System Involving Nuclei and Cytoplasm

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Randomized trial examines prolonged RNA synthesis in a cell-free system, indicating cytoplasm's role.

To develop a cell-free system that allows for prolonged nuclear-directed transcription using nuclei and cytoplasm.
Utilized nuclear and cytoplasmic components from Krebs II ascites tumor cells for experiments.
Measured RNA synthesis rates over time, particularly in the presence and absence of cytoplasm.
Investigated the role of RNA polymerase II in RNA synthesis using alpha-amanitin.
Initial rapid RNA synthesis was observed for 5-10 minutes, followed by a linear rate for 2-3 hours.
In the absence of cytoplasm, no significant RNA synthesis occurred after the initial 10-minute period.
About half of the synthesized RNA was produced by RNA polymerase II.

Wu et al. (1974) studied this question.

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