What question did this study set out to answer?

To investigate whether M2-polarized macrophages and tumor-associated macrophages alter natural killer cell phenotype and antitumor cytotoxicity.

August 4, 2016Journal of Leukocyte Biology

M2-polarized and tumor-associated macrophages alter NK cell phenotype and function in a contact-dependent manner

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Authors

TKTamara KrnetaMcMaster University AGAmy GillgrassMcMaster University Medical Centre SPSophie M. PoznanskiDiscovery Centre

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Implication

Preclinical study demonstrates contact-dependent NK cell suppression by tumor-associated macrophages, highlighting a TGF-beta-mediated therapeutic target.

Key Points

To investigate whether M2-polarized macrophages and tumor-associated macrophages alter natural killer cell phenotype and antitumor cytotoxicity.
In vitro cocultures of murine natural killer (NK) cells with peritoneal or bone marrow-derived M2-polarized macrophages.
Coculture assays using tumor-associated macrophages (TAMs) isolated from spontaneous murine breast tumors to assess cell contact dependency and TGF-β inhibition.
M2 macrophages and TAMs substantially impaired NK cell activation and tumor cell cytotoxicity through direct, contact-dependent mechanisms.
Blockade of macrophage-derived TGF-β restored cytotoxic function in NK cells in contact with M2 macrophages.
TAM exposure induced an exhausted CD27lowCD11bhigh NK cell surface phenotype corresponding to reduced effector activity.

Cite This Study

Krneta et al. (2016) studied this question.

synapsesocial.com/papers/69dc2372ce788f95bfb6508d https://doi.org/10.1189/jlb.3a1215-552r

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Implication

Preclinical study demonstrates contact-dependent NK cell suppression by tumor-associated macrophages, highlighting a TGF-beta-mediated therapeutic target.

M2-polarized and tumor-associated macrophages alter NK cell phenotype and function in a contact-dependent manner

Discussion

Member takes

Implication

Key Points

Cite This Study

M2-polarized and tumor-associated macrophages alter NK cell phenotype and function in a contact-dependent manner

Discussion

Member takes

Implication

Key Points

Cite This Study

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