What question did this study set out to answer?

Determine the atomic structure of the native tick-borne encephalitis virus (TBEV) virion and elucidate the molecular mechanism by which neutralizing antibody 19/1786 prevents infection.

January 24, 2018Nature CommunicationsOpen Access

Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody

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Authors

TFTibor FüzikCentral European Institute of Technology PFPetra FormanováVeterinary Research Institute DRDaniel RůžekMasaryk University

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Implication

Structural analysis reveals the architecture of tick-borne encephalitis virus and antibody binding, highlighting an atypical mechanism that disrupts virus-induced membrane fusion.

Key Points

Determine the atomic structure of the native tick-borne encephalitis virus (TBEV) virion and elucidate the molecular mechanism by which neutralizing antibody 19/1786 prevents infection.
Determined cryo-EM structures of the native mature TBEV virion.
Solved the cryo-EM structure of the TBEV virion in complex with Fab fragments of neutralizing monoclonal antibody 19/1786.
The virion structure indicates that low-pH protonation of histidine side chains induces repulsive forces that disrupt envelope and membrane protein heterotetramers, triggering detachment of envelope ectodomains from the viral membrane.
Monoclonal antibody Fab fragments bind to 120 of the 180 envelope glycoproteins without locking them into native arrangements, instead neutralizing infection by directly inhibiting low-pH-triggered membrane fusion.

Cite This Study

Füzik et al. (2018) studied this question.

synapsesocial.com/papers/69dc32be1eeef32283c0f9dc https://doi.org/10.1038/s41467-018-02882-0

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Implication

Structural analysis reveals the architecture of tick-borne encephalitis virus and antibody binding, highlighting an atypical mechanism that disrupts virus-induced membrane fusion.

Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody

Discussion

Member takes

Implication

Key Points

Cite This Study

Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody

Discussion

Member takes

Implication

Key Points

Cite This Study

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