The striking divergence of ABCB1 mRNA expression and P-glycoprotein protein levels in M1 and M2 macrophages associates with the microRNA miR-21-3p, a regulator of “RNA binding protein, mRNA processing factor”
Compares drug transporter expression in M1 and M2 macrophages, revealing important regulatory interactions.
Key Points
The research aims to investigate the differences in drug transporter expression between M1 and M2 macrophages and how microRNAs regulate these differences.
Differentiation of THP-1 monocytes into M1 and M2 macrophages
Quantitative polymerase-chain reaction for evaluating 84 drug transporter mRNA levels
Western blot for assessing P-glycoprotein protein levels
Evaluation of 46 microRNA species for regulatory roles
Seven transporter genes were lower expressed in M1 compared to M2 macrophages, including ABCG2 and SLCO2B1
Twenty-one transporter genes were higher expressed in M1 macrophages
ABCB1 mRNA was up-regulated threefold in M1, but P-gp protein levels were 2.4-fold lower
Increased expression of miR-21-3p was associated with discrepancies in ABCB1 expression