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April 13, 2026Microbial Cell FactoriesOpen Access

Transcriptomics-guided identification of engineering targets for improving salt tolerance in Saccharomyces cerevisiae

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Authors

MLMao-Ting LiSichuan UniversityLWLinlin WangHarbin University of Science and TechnologyCXCai-Yun XieSichuan University

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Overview

Identifies genetic targets to improve salt tolerance in Saccharomyces cerevisiae, suggesting effective strategies for industrial fermentation.

Key Points

  • The aim is to identify genetic determinants that can enhance salt tolerance in Saccharomyces cerevisiae through transcriptomic analysis.
  • Performed comparative transcriptomic analysis of salt-tolerant strain E-158 and parental strain KF-7 under 1.25 M NaCl stress.
  • Selected two transcription factors (CUP9 and ZNF1) and three functional genes (DAL1, IDP2, CTA1) for validation.
  • Conducted functional validation through overexpression and deletion experiments in the KF-7 strain.
  • Evaluated fermentation performance of engineered strains under high-salinity conditions.
  • Engineered strains significantly outperformed the parental strain under 1.25 M NaCl during fermentation.
  • Overexpression of CTA1 led to a 35.04% increase in glucose consumption and a 45.66% increase in ethanol production after 96 hours.
  • Comparative transcriptomics revealed extensive transcriptional differences between the two strains under salt stress.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69dc88f43afacbeac03eaa85https://doi.org/10.1186/s12934-026-03005-x
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