What question did this study set out to answer?

To characterize the diversity and sequence conservation of T-cell receptor alpha and beta chains specific for the HLA-A2-restricted influenza A virus matrix peptide.

October 15, 1991Proceedings of the National Academy of SciencesOpen Access

Extensive conservation of alpha and beta chains of the human T-cell antigen receptor recognizing HLA-A2 and influenza A matrix peptide.

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Authors

PMPaul MossUniversity Hospitals Birmingham NHS Foundation Trust RMRobert J. MootsEdge Hill University WRWilliam RosenbergCanterbury Christ Church University

Discussion

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Implication

Sequence analysis demonstrates extensive conservation of TCR alpha and beta chains in HLA-A2-restricted influenza-specific T cells, indicating a stereotyped response across unrelated individuals.

Key Points

To characterize the diversity and sequence conservation of T-cell receptor alpha and beta chains specific for the HLA-A2-restricted influenza A virus matrix peptide.
Used polymerase chain reaction (PCR) to amplify and analyze TCR V alpha and V beta gene usage and junctional sequences in cytotoxic T lymphocytes specific for influenza A matrix peptide 57-68.
Compared TCR usage across two unrelated HLA-A2-positive donors and assessed the relative frequency of the identified gene segments within the peripheral blood repertoire.
V alpha and V beta gene usage, as well as distinct junctional sequences, showed extensive sequence conservation across specific cytotoxic T-cell clones.
Two unrelated HLA-A2-positive donors exhibited a shared TCR gene usage pattern, demonstrating that this receptor configuration dominates the anti-influenza A response in HLA-A2 carriers.
The conserved V alpha and V beta gene segments accounted for only a minor fraction of the total unselected peripheral blood TCR pool.

Cite This Study

Moss et al. (1991) studied this question.

synapsesocial.com/papers/69dd069561d4dd8dbb1332cb https://doi.org/10.1073/pnas.88.20.8987

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October 15, 1991Proceedings of the National Academy of SciencesOpen Access

Extensive conservation of alpha and beta chains of the human T-cell antigen receptor recognizing HLA-A2 and influenza A matrix peptide.

View Full Paper

Ask AI

Bookmark

Authors

PMPaul MossUniversity Hospitals Birmingham NHS Foundation Trust RMRobert J. MootsEdge Hill University WRWilliam RosenbergCanterbury Christ Church University

Discussion

Member takes

Implication

Sequence analysis demonstrates extensive conservation of TCR alpha and beta chains in HLA-A2-restricted influenza-specific T cells, indicating a stereotyped response across unrelated individuals.

Key Points

To characterize the diversity and sequence conservation of T-cell receptor alpha and beta chains specific for the HLA-A2-restricted influenza A virus matrix peptide.
Used polymerase chain reaction (PCR) to amplify and analyze TCR V alpha and V beta gene usage and junctional sequences in cytotoxic T lymphocytes specific for influenza A matrix peptide 57-68.
Compared TCR usage across two unrelated HLA-A2-positive donors and assessed the relative frequency of the identified gene segments within the peripheral blood repertoire.
V alpha and V beta gene usage, as well as distinct junctional sequences, showed extensive sequence conservation across specific cytotoxic T-cell clones.
Two unrelated HLA-A2-positive donors exhibited a shared TCR gene usage pattern, demonstrating that this receptor configuration dominates the anti-influenza A response in HLA-A2 carriers.
The conserved V alpha and V beta gene segments accounted for only a minor fraction of the total unselected peripheral blood TCR pool.

Cite This Study

Moss et al. (1991) studied this question.

synapsesocial.com/papers/69dd069561d4dd8dbb1332cb https://doi.org/10.1073/pnas.88.20.8987

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