What question did this study set out to answer?

To identify and map direct binding sites of core snoRNP proteins on U3 snoRNA and pre-rRNA to resolve the structural architecture of the U3 snoRNP complex.

May 30, 2009Proceedings of the National Academy of SciencesOpen Access

Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs

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Authors

SGSander GrannemanWellcome Centre for Cell Biology GKGrzegorz KudlaWestern General Hospital EPElisabeth PetfalskiWellcome Centre for Cell Biology

Discussion

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Implication

Molecular analysis reveals direct binding sites of snoRNP proteins on U3 snoRNA and pre-rRNA in living cells, suggesting a direct role in snoRNA recruitment during ribosome biogenesis.

Key Points

To identify and map direct binding sites of core snoRNP proteins on U3 snoRNA and pre-rRNA to resolve the structural architecture of the U3 snoRNP complex.
Purified cross-linked protein-RNA complexes under highly denaturing conditions following in vitro UV irradiation or in vivo exposure in living cells.
Ligated linkers to isolated RNA fragments, synthesized cDNA, and mapped interaction sites using Sanger sequencing and high-throughput Solexa sequencing.
Identified precise binding sites with high signal-to-noise ratios on U3 snoRNA for the snoRNP proteins Nop1, Nop56, Nop58, and Rrp9.
Demonstrated direct cross-linking between snoRNP proteins and pre-rRNA, showing preferential association at established sites of box C/D snoRNA function.

Cite This Study

Granneman et al. (2009) studied this question.

synapsesocial.com/papers/69dd1ff1d111c0385b35a11f https://doi.org/10.1073/pnas.0901997106

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Implication

Molecular analysis reveals direct binding sites of snoRNP proteins on U3 snoRNA and pre-rRNA in living cells, suggesting a direct role in snoRNA recruitment during ribosome biogenesis.

Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs

Discussion

Member takes

Implication

Key Points

Cite This Study

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