What question did this study set out to answer?

To determine the physiological necessity of the eIF-2alpha kinase PERK in pancreatic regulation, bone development, and postnatal organismal growth.

June 1, 2002Molecular and Cellular BiologyOpen Access

The PERK Eukaryotic Initiation Factor 2α Kinase Is Required for the Development of the Skeletal System, Postnatal Growth, and the Function and Viability of the Pancreas

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Authors

PZPeichuan ZhangWuXi AppTec (China)BMBarbara C. McGrathPennsylvania State University SLSheng’ai LiJilin Agricultural University

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Implication

Animal knockout study uncovers progressive pancreatic degeneration and skeletal dysplasia in Perk-deficient mice, indicating essential roles of PERK in secretory cell viability.

Key Points

To determine the physiological necessity of the eIF-2alpha kinase PERK in pancreatic regulation, bone development, and postnatal organismal growth.
Measured baseline and glucose-stimulated eIF-2alpha phosphorylation levels in nonstressed mouse pancreata.
Engineered and analyzed Perk-knockout (Perk-/-) mice across developmental stages to assess skeletal integrity and pancreatic endocrine and exocrine structure.
Perk-/- mice experienced normal embryonic pancreatic development followed by progressive islet of Langerhans degeneration, leading to beta-cell loss, diabetes mellitus, exocrine digestive enzyme reduction, and massive exocrine apoptosis after postnatal week four.
Knockout mice demonstrated skeletal dysplasias, osteopenia, deficient bone mineralization, and severe postnatal growth failure, all associated with structural abnormalities in the rough endoplasmic reticulum of major secretory cells.

Cite This Study

Zhang et al. (2002) studied this question.

synapsesocial.com/papers/69dd52158557d5ab8f40ce75 https://doi.org/10.1128/mcb.22.11.3864-3874.2002

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Implication

Animal knockout study uncovers progressive pancreatic degeneration and skeletal dysplasia in Perk-deficient mice, indicating essential roles of PERK in secretory cell viability.

The PERK Eukaryotic Initiation Factor 2α Kinase Is Required for the Development of the Skeletal System, Postnatal Growth, and the Function and Viability of the Pancreas

Discussion

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Implication

Key Points

Cite This Study

The PERK Eukaryotic Initiation Factor 2α Kinase Is Required for the Development of the Skeletal System, Postnatal Growth, and the Function and Viability of the Pancreas

Discussion

Member takes

Implication

Key Points

Cite This Study

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