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September 4, 2008Science

TMEM16A, A Membrane Protein Associated with Calcium-Dependent Chloride Channel Activity

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Why the study?

Does TMEM16A knockdown affect calcium-dependent chloride channel activity in bronchial epithelial cells?

Population

Bronchial epithelial cells

Design

Preclinical

Authors

ACAntonella CaputoUniversity of FerraraECEmanuela CaciIstituto Giannina GasliniLFLoretta FerreraIstituto Giannina Gaslini

Discussion

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Implication

Hypothesis-generating for TMEM16A targeting in epithelial disorders; human validation required before clinical consideration.

Structured PICO

Does TMEM16A knockdown affect calcium-dependent chloride channel activity in bronchial epithelial cells?

P
Population
Bronchial epithelial cells
I
Intervention
Treatment with interleukin-4 (IL-4) and transfection with specific small interfering RNA against TMEM16A
O
Outcome
Calcium-dependent chloride current measured with halide-sensitive fluorescent proteins, short-circuit current, and patch-clamp techniquessurrogate

Identifies TMEM16A as an intrinsic constituent of the calcium-dependent chloride channel, providing a new target for basic research and drug development.

Cite This Study

Caputo et al. (2008) studied this question.

synapsesocial.com/papers/69dd5ca27808b00a4799d060https://doi.org/10.1126/science.1163518
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular and Functional Characterization of a Calcium-sensitive Chloride Channel from Mouse Lung1998 · 140 citations
  2. 2The vitelliform macular dystrophy protein defines a new family of chloride channels2002 · 475 citations
  3. 3IL-4 Is a Potent Modulator of Ion Transport in the Human Bronchial Epithelium In Vitro2002 · 137 citations
  4. 4An Immortalized Cystic Fibrosis Tracheal Epithelial Cell Line Homozygous for the ΔF508 CFTR Mutation1993 · 117 citations
  5. 5Extracellular 2‐chloroadenosine and ATP stimulate volume‐sensitive Cl− current and calcium mobilization in human tracheal 9HTEo− cells1992 · 30 citations