What question did this study set out to answer?

To evaluate the efficacy of incorporating acid, rose bengal, and streptomycin into agar media for selectively enumerating soil fungi.

March 1, 1950Soil Science

Use of Acid, Rose Bengal, and Streptomycin in the Plate Method for Estimating Soil Fungi

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Authors

JMJames P. MartinVietnam Academy of Science and Technology

Discussion

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Implication

Laboratory evaluation demonstrates selective media formulations using acid, rose bengal, and streptomycin for soil fungi quantification, highlighting improved methods for fungal colony enumeration.

Key Points

To evaluate the efficacy of incorporating acid, rose bengal, and streptomycin into agar media for selectively enumerating soil fungi.
Employed the agar plate dilution method to isolate and enumerate fungal populations from soil.
Evaluated selective inhibitors, including medium acidification, rose bengal dye, and streptomycin, to suppress bacterial growth and restrict fungal colony spread.
Incorporation of rose bengal and streptomycin suppressed bacterial contamination more effectively than acidification alone.
The combination restricted the size of spreading fungal colonies, enabling more distinct and accurate colony enumeration on agar plates.

Cite This Study

James P. Martin (1950) studied this question.

synapsesocial.com/papers/69ddd05b7ed287395e55835a https://doi.org/10.1097/00010694-195003000-00006

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Implication

Laboratory evaluation demonstrates selective media formulations using acid, rose bengal, and streptomycin for soil fungi quantification, highlighting improved methods for fungal colony enumeration.

Use of Acid, Rose Bengal, and Streptomycin in the Plate Method for Estimating Soil Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

Use of Acid, Rose Bengal, and Streptomycin in the Plate Method for Estimating Soil Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

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