Regulatory B cells (Bregs) maintain immune homeostasis through anti-inflammatory cytokine production, yet their role in systemic lupus erythematosus (SLE) remains poorly understood. This study evaluated CD19+CD24highCD27+ B cell frequencies and serum interleukin-10 (IL-10) and interleukin-35 (IL-35) in 40 SLE patients compared to 25 controls. Active SLE patients showed significantly reduced CD19+CD24highCD27+ B cells (23.20×106) compared to controls (56.40×106, p<0.001). IL-35 levels were decreased in active disease (54.04±8.10 pg/ml) versus inactive disease (83.38±20.05 pg/ml) and controls (89.04±10.86 pg/ml, p<0.001). IL-10 concentrations were elevated in active patients (4.44±1.27 pg/ml) compared to inactive and controls (1.95±0.56 and 2.03±0.61 pg/ml, p<0.001). Strong correlations were observed between regulatory B cell counts and SLEDAI scores (r=-0.547, p<0.001). In conclusion, active SLE is characterized by impaired regulatory B cell populations and dysregulated cytokine profiles, with reduced IL-35 and elevated IL-10 levels correlated with disease activity.
Abdeen et al. (Wed,) studied this question.