What question did this study set out to answer?

To characterize shifts in the subsets and transcriptomic profiles of IL-10-producing regulatory B cells across stages of disease progression in lupus-prone mice.

December 14, 2023Frontiers in ImmunologyOpen Access

Single-cell RNA sequencing analysis reveals the heterogeneity of IL-10 producing regulatory B cells in lupus-prone mice

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Authors

ADAndrea R. DaamenAmpel BioSolutions (United States)RARazan AlajoleenVirginia Tech AGAmrie C. GrammerAmpel BioSolutions (United States)

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Implication

Single-cell transcriptomic profiling reveals lineage shifts and functional depletion of regulatory B cells in lupus-prone mice, highlighting cellular mechanisms of disease progression.

Key Points

To characterize shifts in the subsets and transcriptomic profiles of IL-10-producing regulatory B cells across stages of disease progression in lupus-prone mice.
Conducted single-cell RNA sequencing (scRNA-seq) on splenic regulatory B cells (Bregs) from lupus-prone mice at pre-disease and active disease stages.
Validated transcriptome-identified alterations across Breg subsets using phenotypic analysis.
Active disease was characterized by a loss of marginal zone lineage Bregs, an expansion of plasmablast/plasma cell lineage Bregs, and elevated inflammatory gene signatures compared to the pre-disease stage.
The frequencies of both marginal zone Bregs and plasmablasts/plasma cells actively expressing immunosuppressive IL-10 were significantly decreased during active disease.

Cite This Study

Daamen et al. (2023) studied this question.

synapsesocial.com/papers/6a19cdf13f3ec013f0df15c8 https://doi.org/10.3389/fimmu.2023.1282770

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Implication

Single-cell transcriptomic profiling reveals lineage shifts and functional depletion of regulatory B cells in lupus-prone mice, highlighting cellular mechanisms of disease progression.

Single-cell RNA sequencing analysis reveals the heterogeneity of IL-10 producing regulatory B cells in lupus-prone mice

Discussion

Member takes

Implication

Key Points

Cite This Study

Single-cell RNA sequencing analysis reveals the heterogeneity of IL-10 producing regulatory B cells in lupus-prone mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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