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Isolate and characterize novel monocyte chemotactic factors secreted by cytokine-stimulated human osteosarcoma cells.

July 1, 1992The Journal of Experimental MedicineOpen Access

Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family.

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Authors

JDJo Van DammeKU Leuven PPPaul ProostUmicore (Belgium)JLJean‐Pierre LenaertsRega Institute for Medical Research

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Implication

Laboratory study reveals two novel tumor-derived chemokines that selectively recruit monocytes in vivo, suggesting complex roles in tumor-immune interactions.

Key Points

Isolate and characterize novel monocyte chemotactic factors secreted by cytokine-stimulated human osteosarcoma cells.
Purified secreted proteins from cytokine-stimulated MG-63 human osteosarcoma cells using heparin-Sepharose chromatography, cation-exchange FPLC, and reverse-phase HPLC.
Identified primary peptide structures by sequencing fragments of the isolated 7.5-kD and 11-kD proteins.
Assessed in vitro chemotactic specificity against monocytes and neutrophils, and measured in vivo monocyte recruitment following intradermal injection in rabbits.
Identified two novel chemokines, designated MCP-2 (7.5 kD) and MCP-3 (11 kD), exhibiting structural homology to MCP-1 and conserved cysteine motifs.
Demonstrated that MCP-2 and MCP-3 exhibit specific chemotactic activity comparable to MCP-1, selectively attracting monocytes while showing no chemotactic effect on neutrophils in vitro.
Observed selective in vivo recruitment of monocytes following intradermal injection in rabbit models.

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Damme et al. (1992) studied this question.

synapsesocial.com/papers/69debcaffd84e72eb2558bb1 https://doi.org/10.1084/jem.176.1.59

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Implication

Laboratory study reveals two novel tumor-derived chemokines that selectively recruit monocytes in vivo, suggesting complex roles in tumor-immune interactions.

Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family.

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Implication

Key Points

Cite This Study

Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family.

Discussion

Member takes

Implication

Key Points

Cite This Study

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