What question did this study set out to answer?

To investigate the expression and in vivo requirement of the transcription factor scleraxis during heart valve development and extracellular matrix remodeling.

September 19, 2008Circulation ResearchOpen Access

Scleraxis Is Required for Cell Lineage Differentiation and Extracellular Matrix Remodeling During Murine Heart Valve Formation In Vivo

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Population

Murine models including scx-GFP reporter mice and mice null for scx (Scx) evaluated from embryonic day 15.5…

Comparison

Genetic deletion of scleraxis (Scx(-/-)) vs Wild-type mice (implied)

Design

Preclinical

Follow-up

Embryonic day 15.5 through postnatal stages

Authors

ALAgata LevayUniversity of Miami JPJacqueline D. PeacockCincinnati Children's Hospital Medical Center YLYinhui LuWellcome Centre for Cell-Matrix Research

Discussion

Member takes

Implication

Scleraxis emerges as essential for valve matrix remodeling; extends developmental insights but leaves open human disease translation.

Key Points

To investigate the expression and in vivo requirement of the transcription factor scleraxis during heart valve development and extracellular matrix remodeling.
Tracked scleraxis expression across embryonic day 15.5 through postnatal stages using Scx-GFP reporter mice.
Assessed valve morphology, precursor cell lineage differentiation, matrix gene expression, and collagen ultrastructure in scleraxis-null (Scx-/-) mice.
Scx(-/-) mice displayed significantly thickened heart valve leaflets beginning at embryonic day 17.5 and excessive annular collagen deposition in the atrioventricular junction.
Mutant valves demonstrated impaired matrix remodeling and differentiation, marked by reduced collagen type XIV, elevated Sox9 and cartilage genes, persistent Msx1 and Snai1 expression, and disrupted collagen fiber ultrastructure.

Structured PICO

Population

Murine models including scx-GFP reporter mice and mice null for scx (Scx(-/-)) evaluated from embryonic day 15.5 through postnatal stages

Intervention

Genetic deletion of scleraxis (Scx(-/-))

Comparator

Wild-type mice (implied)

Outcome

Heart valve structure thickness, cell lineage differentiation, and extracellular matrix organizationsurrogate

Scleraxis is required in vivo for proper cell lineage differentiation and extracellular matrix distribution during heart valve remodeling.

Cite This Study

Levay et al. (2008) studied this question.

synapsesocial.com/papers/69df0beed85e58e37b7a0f85 https://doi.org/10.1161/circresaha.108.177238

Topics

Valvular heart disease

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Scleraxis Is Required for Cell Lineage Differentiation and Extracellular Matrix Remodeling During Murine Heart Valve Formation In Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Scleraxis Is Required for Cell Lineage Differentiation and Extracellular Matrix Remodeling During Murine Heart Valve Formation In Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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