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April 15, 2026Small

Extracellular Fluorescence Recording of BacFlash Dynamics Reveals Two Distinct Modes of Proton Extrusion

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Authors

JGJ. GaoYZYaoyao ZhangHSHua Su

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Overview

Experimental findings reveal distinct proton extrusion modes in BacFlash processes, indicating a novel bacterial regulation mechanism.

Key Points

  • This research aims to understand BacFlash dynamics by investigating proton extrusion mechanisms in bacteria.
  • Developed an extracellular label-free fluorescence imaging technique.
  • Confined individual E. coli cells in femtoliter-scale microwells.
  • Quantified transient proton extrusion at the single-cell level under various conditions.
  • Measured proton release rates of approximately 10^5 to 10^6 protons per cell per second.
  • Identified two distinct forms of BacFlash with significant differences in proton efflux rates and durations.
  • Showed modulation of BacFlash dynamics by ultraviolet stimulation.

Cite This Study

Gao et al. (2026) studied this question.

synapsesocial.com/papers/69df2ba0e4eeef8a2a6b09b2https://doi.org/10.1002/smll.202513944
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