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April 15, 2026Scientific ReportsOpen Access

3D models for oral inflammation using gingival fibroblasts, lipopolysaccharides and hypoxia

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Authors

AAAnne Eriksson AggerJHJana HlinkováJRJanne Elin Reseland

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Overview

3D models assess inflammation and hypoxia effects on tissue structure in gingival fibroblasts, implying insights into periodontal disease.

Key Points

  • The research aims to develop a 3D model to analyze the effects of hypoxia and inflammatory signals on fibroblast behavior in oral inflammation.
  • Created a 3D spheroid model using human gingival fibroblasts.
  • Applied E. coli lipopolysaccharides and hypoxic conditions.
  • Analyzed changes in spheroid structure and extracellular matrix.
  • Conducted gene expression profiling and secretome analysis.
  • Hypoxia alone altered spheroid morphology, creating smaller aggregates.
  • Combining hypoxia and inflammation caused significant structural disruptions.
  • Observed suppression of genes related to extracellular matrix and adhesion.
  • Increased production of pro-inflammatory cytokines and VEGFα was noted.
  • Cell viability remained largely unchanged despite these alterations.

Cite This Study

Agger et al. (2026) studied this question.

synapsesocial.com/papers/69df2c9ee4eeef8a2a6b1ca0https://doi.org/10.1038/s41598-026-47648-7
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