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August 7, 2026BMC Oral HealthOpen Access

Effects of periodontal pathobiont infection on a 3D mucosa model

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Authors

PPPearly PerumpallilHWHedwig WariwodaDBDaniel Baumhoer

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Overview

Randomized trial examines changes in mucosal integrity and cytokine response from bacterial infection.

Key Points

  • This study aims to develop a 3D mucosa model to investigate the effects of periodontal pathobiont infections on epithelial integrity and immune response.
  • Developed a 3D mucosa model using human gingival fibroblasts and epithelial progenitor cells.
  • Cultured cells in transwell inserts for 28 days with media transitions and assessed tissue organization and barrier function.
  • Examined responses to bacterial exposure with P. gingivalis and F. nucleatum and measured cytokine levels via ELISA.
  • The 3D model showed 4–6 cohesive epithelial layers with a functional barrier as confirmed by minimal dextran-FITC translocation.
  • 16-hour exposure to the dual-species biofilm led to wider intercellular spaces and partial loss of epithelial cohesion.
  • 24-hour exposure resulted in visible bacterial invasion and modulation of cytokines IL-8, IL-6, and IL-1β in response to infection.

Cite This Study

Perumpallil et al. (2026) studied this question.

synapsesocial.com/papers/6a758bd0847ab6d26c01f4e1https://doi.org/10.1186/s12903-026-09492-z
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