Purpose: To identify the main flavonoid in Adinandra nitida leaf, employ response surface methodology to optimize its ultrasonic-assisted extraction, and determine the antioxidant activity of the obtained extract.Methods: The main flavonoid in Adinandra nitida leaf was obtained by traditional solvent extraction and recrystallization methods, and identified by ultraviolet-visible spectroscopy (UV), Fourier transform infrared spectroscopy (FT-IR), electrospray ionization-mass spectrometry (ESI-MS), nuclear magnetic resonance spectroscopy (NMR). By using response surface methodology, the effects of extraction time, ethanol concentration and ultrasonic frequency on flavonoid yield were investigated and optimal conditions determined. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power of the obtained extract was also examined.Results: The main flavonoid in Adinandra nitida leaf was identified as camellianin A. Optimal extraction conditions were as follows: extraction time, 30.25 min; ethanol concentration, 63.84 %; and ultrasonic frequency, 45 KHz. The mean experimental flavonoid yield under optimum conditions was 84.52 ±1.65 %, which agreed with the predicted value of 83.02 %. The obtained flavonoid extract was an effective scavenger of DPPH radicals with IC50 of 0.02 mg/mL. A linear correlation between concentration of the flavonoid extract and reducing power was observed with a coefficient of r2 = 0.9867.Conclusion: By using ultrasonic-assisted extraction, the main flavonoid in Adinandra nitida leaf can be obtained at a high yield. The flavonoid displays a strong DPPH radical scavenging activity and reducing power, which makes it potentially useful in the food and pharmaceuticals industries.
No takes yet. Share an insight, caveat, or question.
Liu et al. (2014) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: