What question did this study set out to answer?

Investigate how varying concentrations of glucose regulate the transcriptional induction of hexose transporter genes (HXT1 to HXT4) in Saccharomyces cerevisiae.

March 1, 1995Molecular and Cellular BiologyOpen Access

Three Different Regulatory Mechanisms Enable Yeast Hexose Transporter ( HXT ) Genes To Be Induced by Different Levels of Glucose

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Investigate how varying concentrations of glucose regulate the transcriptional induction of hexose transporter genes (HXT1 to HXT4) in Saccharomyces cerevisiae.
Assayed HXT1 through HXT4 transcriptional expression in Saccharomyces cerevisiae exposed to varying glucose concentrations.
Evaluated gene expression changes in genetic mutant strains, including grr1, rgt1, ssn6, hxk2, reg1, tup1, and mig1.
Glucose induced HXT transcription 10- to 300-fold across three distinct modes: concentration-independent (HXT3), low-glucose-specific with high-glucose repression (HXT2, HXT4), and high-glucose-only (HXT1).
Basal repression in the absence of glucose depended on Rgt1p and Ssn6p, with Grr1p acting as a positive regulator that relieves Rgt1p-mediated repression in response to glucose.
High-glucose repression of HXT2 and HXT4 and maximal high-glucose induction of HXT1 both required glucose repression pathway components, including Hxk2p and Reg1p.

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Implication

Experimental analysis reveals three distinct glucose-dependent induction mechanisms for hexose transporter genes in yeast, indicating shared regulatory components between induction and repression.

Cite This Study

Özcan et al. (1995) studied this question.

synapsesocial.com/papers/69e14d68f608f0c4ffa1a164 https://doi.org/10.1128/mcb.15.3.1564

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Implication

Experimental analysis reveals three distinct glucose-dependent induction mechanisms for hexose transporter genes in yeast, indicating shared regulatory components between induction and repression.

Three Different Regulatory Mechanisms Enable Yeast Hexose Transporter ( HXT ) Genes To Be Induced by Different Levels of Glucose

Key Points

Discussion

Implication

Cite This Study

Three Different Regulatory Mechanisms Enable Yeast Hexose Transporter ( HXT ) Genes To Be Induced by Different Levels of Glucose

Key Points

Discussion

Implication

Cite This Study

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