Antianemic Potential of Artocarpus chaplasha Seeds in Phenyl Hydrazine–Induced Hemolytic Anemia: Evidence From Swiss Albino Mice and Molecular Docking Studies

Background and Aim Anemia affects billions, primarily young women and men. Taking steroids and immunosuppressants might cause side effects. So recent studies are focusing on natural hematinic agents to cure anemia. This study examined Artocarpus chaplasha seeds against phenyl hydrazine–induced hemolytic anemia in Swiss albino mice. Methods To induce anemia, mice administered 60 mg/kg phenyl hydrazine intraperitoneally for 2 days. On Day 3, decreased RBC, hemoglobin, and hematocrit levels revealed anemia. Mice were orally treated with A. chaplasha seed methanol extract. Vitamin B 12 was the reference standard. Post 200 and 400 mg/kg test extract administration, blood was taken for hematological analysis on Day 15. All in vivo results were analyzed and supported through in silico approach. Results Compared to positive control group, A. chaplasha seeds doses of 200 and 400 mg/kg significantly ( ∗∗ p < 0.01) increased RBC levels. Hemoglobin levels considerably increased with 200 mg/kg ( ∗ p < 0.05) and 400 mg/kg ( ∗∗ p < 0.01). The 200 and 400 mg/kg doses significantly elevated hematocrit (PCV) levels ( ∗ p < 0.05 and ∗∗∗ p < 0.001). Furthermore, in silico molecular docking studies showed significant binding affinity of the compounds against the receptor protein (PDB ID: 2HHB). With −7.2 kcal/mol, neophytadiene bound strongly. Conclusion The in vivo activity demonstrate increase in the RBC level, and docking score shows comparability to the positive control, folinic acid with a score of −6.9 kcal/mol. Thus, the A. chaplasha seed may be a promising natural anemic treatment.