Identification of Shared Regulators of IgE ‐ and MRGPRX2 ‐Dependent Degranulation in Human Mast Cells

The data that support the findings of this study are available from the corresponding author upon reasonable request. Figure S1: (A) Heatmap illustrating the expression levels of 85 shared significantly upregulated genes with uncertain functions in mast cell activation following stimulation of mast cells from donors 1 and 2 (D1 and D2) with various stimuli for 1 h. (B) qPCR validation of RGS1 and GEM induction in PBMC-derived human mast cells stimulated for 1 h with anti-IgE (n = 5 independent donors) or Substance P (n = 2 independent donors). Expression is shown as relative mRNA levels normalized to ACTB and plotted relative to Veh-treated cells. Bars show mean ± SEM. (C) Expression heatmap comparing shared upregulated genes after 1 h of stimulation vs. shared upregulated genes after 6 h of stimulation in D1 and D2. Data S1: Supporting Information. Table S1: RPKM expression matrix for all measured genes comparing 1-h and 6-h stimulation for each tested stimulus. Table S2: Log2 expression values of shared upregulated cytokines, chemokines, TFs, and known negative regulators of mast cell activation. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.