Recent advances in mapping protein networks regulated by post-translational modifications via proximity tagging

Recent platform innovations are now enabling the resolution of the different ways protein–protein interactions (PPIs) are wired by post-translational modifications (PTMs). Such PTM-aware interactome maps are facilitating the systematic cataloguing of context-dependent network changes. This review highlights developments from 2023 to 2025, focusing on acetylation, methylation, phosphorylation, ubiquitination, redox-regulated cysteine networks, and glycosylation, and outlines analytical and biological frontiers. Together, these advancements are shifting PTM-informed proximal interactome mapping toward mechanism-grounded, actionable frameworks.