Aedes aegypti is a mosquito species responsible for transmission of dengue fever. WHO stated the best way to control the transmission of dengue virus is to fight the vector mosquito that cause disease. In present work we evaluated the potential larvicide from Cestrum nocturnum and Sapindus mukorossi leaves. Methanol extract showing highest activity of larvicide were separated and fractionated using chromatographic techniques and bioassay guided fractionation. Bioactive fractions were subjected for LC50 evaluation on 3rd instars larvae of Aedes aegypti according to WHO protocol. Using hexane ethyl acetate solvent system, CN-3 and SM-5 fractions are separated containing larvicide with LC50 7.2 μg/ml and 18.3 μg/ml respectively for 24h. These fractions will be further characterized to identify the compound and biologically evaluated for mode of action on targeted organism. This study further lead to produce less expensive and safe compound to control mosquitoes in India.
Chetan Jawale (Sat,) studied this question.