Development of a surface plasmon resonance sensor for assessing infertility based on Anti-Mullerian hormone levels

Anti-Müllerian hormone (AMH) is a key biomarker for fertility assessment, but current diagnostic methods lack cost-effectiveness. Plasmonic biosensors can effectively detect low-concentration protein interactions. This study developed a sensitive, label-free surface plasmon resonance (SPR) sensor for AMH detection. Protein G was used to bind anti-AMH antibodies onto a gold chip coated with 11-MUA. Various AMH concentrations were measured to create a standardized graph, establishing limits of detection (LOD) and quantification (LOQ). The biosensor also tested patient serum samples for AMH detection, comparing the results with those obtained from enzyme-linked immunosorbent assay (ELISA) and localized surface plasmon resonance (LSPR) using silica@Au-NRs with anti-AMH antibodies. The detection results indicated a high sensitivity of the nanobiosensors, with LOD and LOQ for the gold chip at 0.6 ng/mL and 1.78 ng/mL, respectively, closely matching those of ELISA and silica@Au-NRs. Additionally, SPR-based biosensors reduce detection costs and measurement time, making them strong candidates for integration into diagnostic kits.