Identification of the Novel HLA‐A*33:278 Allele in a Chinese Individual by Sanger Dideoxy Nucleotide Sequencing

Key Points

• To identify and characterize the novel HLA-A*33:278 allele.
• Utilized Sanger dideoxy nucleotide sequencing to analyze HLA alleles.
• Focused on nucleotide substitution in codon 185 in exon 4.
• Compared newly identified allele with HLA-A*33:03:01:01.
• Discovered a novel allele, HLA-A*33:278, differing from HLA-A*33:03:01:01 by one nucleotide.
• The nucleotide substitution occurred at codon 185 in exon 4.