What question did this study set out to answer?

The aim was to develop a convenient in vitro bioassay to measure bioactive FSH without using live animals.

April 27, 2026Open Access

In vitro bioassay for human FSH based on L cells transfected with recombinant rat FSH receptor: validation of a model system.

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Randomized trial validates an in vitro bioassay for FSH bioactivity in human serum, highlighting its efficiency and sensitivity.

The aim was to develop a convenient in vitro bioassay to measure bioactive FSH without using live animals.
Developed L cell line expressing rat FSH receptor for high-affinity FSH binding.
Assessed the effect of heating human serum to reduce interference in cAMP accumulation.
Analyzed serum from male infertility patients and menstruating women using the novel bioassay.
Established high correlation (r > 0.8; P < 0.01) of the new assay with existing tests for FSH bioactivity.
Detected midcycle FSH peak in 25 normal menstruating women.
Achieved close correlation (r = 0.90; P < 0.01) between the novel bioassay and a fluoroimmunoassay in postmenopausal women.

T et al. (1994) studied this question.

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