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The aim is to develop a sensitive bioassay for measuring LH and hCG levels in human serum using rat Leydig cells.

InVitroBioassay of LH in Human Serum: The Rat Interstitial Cell Testosterone (RICT) Assay

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The aim is to develop a sensitive bioassay for measuring LH and hCG levels in human serum using rat Leydig cells.
Utilized collagenase-dispersed rat Leydig cells for bioassay
Performed assays with a variety of gonadotropins including human and animal LH
Ensured assay precision through controlled incubation conditions and measurement protocols.
Sensitive detection limits of 50 muU for hMG and 20 muU for hCG were achieved.
Parallel dose-response curves demonstrated for all tested gonadotropins.
LH levels in normal subjects consistently measurable with a good precision of +/- 10% within-assay variation.

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Randomized trial demonstrates a new bioassay for LH in human serum, implying enhanced measurement precision.

Dufau et al. (1976) studied this question.

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