What question did this study set out to answer?

Investigate pericyte dysfunction during muscle disuse and evaluate whether extracellular vesicles from primed pericytes can restore skeletal muscle mass in aged models.

March 23, 2022The Journal of PhysiologyOpen Access

Development of a cell‐free strategy to recover aged skeletal muscle after disuse

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Authors

YWYu‐Fu WuInstitute of Molecular Biology, Academia Sinica ETEduardo A. De La TobaUniversity of Illinois Urbana-Champaign SDSvyatoslav DvoretskiyUniversity of Illinois Urbana-Champaign

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Implication

Experimental study demonstrates that primed pericyte extracellular vesicles restore myofibre size in aged mice after disuse, highlighting a novel cell-free therapy for muscle recovery.

Key Points

Investigate pericyte dysfunction during muscle disuse and evaluate whether extracellular vesicles from primed pericytes can restore skeletal muscle mass in aged models.
Performed single-cell RNA sequencing and functional assays to characterize pericyte antioxidant responses during immobilization and recovery in mouse skeletal muscle.
Treated healthy donor pericytes with hydrogen peroxide in vitro to generate primed small extracellular vesicles (sEVs) for administration in disused adult and aged mice.
Conducted proteomic profiling on primed sEVs to identify protein cargo driving muscle recovery.
Single-cell RNA sequencing showed that skeletal muscle pericytes lose their capacity to synthesize antioxidants during disuse and subsequent recovery.
Administration of hydrogen peroxide-primed pericyte-derived sEVs effectively restored myofibre size and supported extracellular matrix remodeling in both adult and aged mice post-disuse.
Proteomic assessment identified 11 differentially regulated proteins in primed sEVs associated with antioxidant activity, anti-inflammatory mechanisms, and extracellular matrix composition.

Cite This Study

Wu et al. (2022) studied this question.

synapsesocial.com/papers/69ef6ccc4ab5feda0eb2552e https://doi.org/10.1113/jp282867

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Implication

Experimental study demonstrates that primed pericyte extracellular vesicles restore myofibre size in aged mice after disuse, highlighting a novel cell-free therapy for muscle recovery.

Development of a cell‐free strategy to recover aged skeletal muscle after disuse

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Implication

Key Points

Cite This Study

Development of a cell‐free strategy to recover aged skeletal muscle after disuse

Discussion

Member takes

Implication

Key Points

Cite This Study

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