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April 30, 2026˜Die œPharmazieOpen Access

Molecular mechanisms of the anti-inflammatory effect of sinomenine on atopic dermatitis

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Authors

SLShiwei LinMWMinglei WeiWZWei Zhu

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Implication

In vitro research reveals sinomenine's dose-dependent inhibition of inflammatory cytokines in atopic dermatitis models, indicating therapeutic potential.

Key Points

  • This research investigates how sinomenine affects inflammation in atopic dermatitis at the molecular level.
  • Established a cell model of atopic dermatitis using RAW264.7 cells stimulated with LPS.
  • Treated cells with varying concentrations of sinomenine and assessed cell viability by MTT assay.
  • Measured pro-inflammatory cytokines, including interleukin-1β and tumor necrosis factor-α, using ELISA.
  • Analyzed NO production through a nitrate/nitrite assay kit and gene/protein expressions via qRT-PCR and western blot.
  • Sinomenine significantly reduced the levels of pro-inflammatory cytokines in a dose-dependent manner.
  • LPS-induced increase in NO production was inhibited by sinomenine treatment.
  • Expression levels of iNOS and COX2 decreased upon sinomenine pretreatment.
  • Activation of the p38MAPK-NF-κB pathways was suppressed by sinomenine administration.

Cite This Study

Lin et al. (2018) studied this question.

synapsesocial.com/papers/69f2a49d8c0f03fd6776399dhttps://doi.org/10.31083/ph.2018.8047
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sinomenine Regulates the TRIM32 / IRF1 / TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis2026
  2. 2Antioxidant Activity and the Therapeutic Effect of Sinomenine Hydrochloride-Loaded Liposomes-in-Hydrogel on Atopic Dermatitis2024 · 12 citations
  3. 3Sinomenine treats rheumatoid arthritis by inhibiting MMP9 and inflammatory cytokines expression: bioinformatics analysis and experimental validation2024 · 22 citations
  4. 4Integrated Network Pharmacology, Molecular Docking, and Experimental Validation to Explore Potential Mechanisms of Sinomenine in the Treatment of Osteoarthritis2024 · 1 citations
  5. 5Chemical proteomics reveals sinomenine’s anti-inflammatory mechanism through serum protein covalent modification2026 · 1 citations