June 15, 2017SHILAP Revista de lepidopterología109 citationsOpen Access

Protective effect of rutin supplementation against cisplatin-induced Nephrotoxicity in rats

AAAli AlhoshaniKing Saud University MHMohamed M. HafezAhram Canadian University SHSufia HusainKing Saud Medical City

Structured PICO

Does rutin supplementation prevent cisplatin-induced nephrotoxicity in male Wistar albino rats?

Population

40 male Wistar albino rats

Intervention

Rutin 30 mg/kg orally plus single dose 5 mg/kg cisplatin i.p.

Comparator

Single dose 5 mg/kg cisplatin i.p. alone, rutin 30 mg/kg alone, and control group (2.5 ml/kg i.p.)

Outcome

Nephrotoxicity assessed by BUN, serum creatinine, MDA, histopathology, and gene expression of inflammatory markers (JNK, MKK4, MKK7, P38, TNF-α, TRAF2, IL-1-α)surrogate

Rutin supplementation attenuates cisplatin-induced nephrotoxicity in rats by decreasing oxidative stress and inhibiting inflammatory signaling pathways.

Abstract

BACKGROUND: Cisplatin (CP) is commonly used in the treatment of different types of cancer but nephrotoxicity has been a major limiting factor. Therefore, the present study aimed to study the possible protective effect of rutin against nephrotoxicity induced by cisplatin in rats. METHODS: Forty male Wistar albino rats were randomly divided into 4 groups. Rats of group 1 control group intraperitoneal (i.p.) received 2.5 ml/kg, group 2 CP group received single dose 5 mg/kg cisplatin i.p. group 3 rutin group orally received 30 mg/kg rutin group 4 (CP plus rutin) received CP and rutin as in group 2 and 3. Kidneys were harvested for histopathology and for the study the gene expression of c-Jun N-terminal kinases (JNK), Mitogen-activated protein kinase 4 (MKK4), MKK7, P38 mitogen-activated protein kinases (P38), tumor necrosis factors alpha (TNF-α), TNF Receptor-Associated Factor 2 (TRAF2), and interleukin-1 alpha (IL-1-α). RESULTS: The cisplatin single dose administration to rats induced nephrotoxicity associated with a significant increase in blood urea nitrogen (BUN) and serum creatinine and significantly increase Malondialdehyde (MDA) in kidney tissues by 230 ± 5.5 nmol/g compared to control group. The animal treated with cisplatin showed a significant increase in the expression levels of the IL-1α (260%), TRFA2 (491%), P38 (410%), MKK4 (263%), MKK7 (412%), JNK (680%) and TNF-α (300%) genes compared to control group. Additionally, histopathological examination showed that cisplatin-induced interstitial congestion, focal mononuclear cell inflammatory, cell infiltrate, acute tubular injury with reactive atypia and apoptotic cells. Rutin administration attenuated cisplatin-induced alteration in gene expression and structural and functional changes in the kidney. Additionally, histopathological examination of kidney tissues confirmed gene expression data. CONCLUSION: The present study suggested that the anti-oxidant and anti-inflammatory effect of rutin may prevent CP-induced nephrotoxicity via decreasing the oxidative stress, inhibiting the interconnected ROS/JNK/TNF/P38 MAPK signaling pathways, and repairing the histopathological changes against cisplatin administration.

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