Enhancing the Thermostability and Catalytic Activity of a Multicopper Oxidase from Bacillus megaterium for Aflatoxin B 1 and Zearalenone Degradation

Key Points

• The aim is to enhance the thermostability and catalytic activity of CueO for mycotoxin degradation.
• Utilized a multicopper oxidase enzyme derived from Bacillus megaterium.
• Tested enzyme efficiency in degrading aflatoxin B1 and zearalenone.
• Assessed catalytic activity under varying temperature conditions.
• CueO exhibited significant degradation of aflatoxin B1 and zearalenone.
• Enhanced thermostability resulted in improved catalytic activity under elevated temperatures.
• Enzyme achieved a degradation rate of 85% for aflatoxin B1 after 60 minutes at optimal conditions.