HPLC and Mass Spectrometric Analysis of Carotenoids from Mango

β-Carotene (all-trans), β-cryptoxanthin (all-trans and cis), zeaxanthin (all-trans), luteoxanthin isomers, violaxanthin (all-trans and cis), and neoxanthin (all-trans and cis) were identified in mango cultivar Keitt by means of HPLC data (retention time, co-injection, and chemical reactions). Mass spectrometry (molecular ion, fragmentation pattern, m/z relative intensity) confirmed the identity of these carotenoids (not the geometric form); additionally, lutein and mutatochrome were detected when a large amount of sample was utilized. The quantitative composition was determined by HPLC, Sudan I being used as internal standard. all-trans-Violaxanthin (21.1 ± 2.9 μg/g), all-trans-β-carotene (15.1 ± 1.5 μg/g), and a cis-violaxanthin (10.1 ± 0.2 μg/g), tentatively identified as 9-cis, were the principal carotenoids.