GEO uploader: simplifying the data deposition in the GEO repository

Background The Gene Expression Omnibus (GEO) (Clough and Barrett in: methods in molecular biology, Clifton, 2016) repository requires complex multistep submissions involving metadata preparation, FTP uploads, and MD5 validation. Current manual processes are error-prone, time-consuming, and require significant bioinformatics expertise, creating barriers for many researchers. Results We present GEO Uploader, a web-based tool that automates the entire GEO submission workflow through an intuitive interface. The application reduces the submission initiation time from 2–3 h to under 20 s by automating file uploads, MD5 calculations, and metadata template population. Key features include parallel processing of uploads and checksum calculations, automated error prevention through template-based metadata completion, real-time progress tracking, and support for complex submission structures. Deployment across 30 + users with 50 + upload sessions, including datasets exceeding hundreds of gigabytes, demonstrates practical utility and reliability in research environments. Conclusion GEO Uploader significantly reduces the technical barrier for GEO submissions while minimizing errors through comprehensive automation. The tool supports data sharing by enabling researchers without specialized bioinformatics expertise to complete submissions independently. Available as open-source software with multiuser deployment capabilities, GEO Uploader represents a substantial improvement in research data sharing accessibility and supports broader adoption of open science practices in the genomics community.