The effect of swimming training and royal jelly consumption at different doses on IL-6 and LPS-induced IKB-Z gene expression in mice with benzo[a]pyrene -induced lung cancer

Introduction: Lung cancer is a major threat to global health and well-being, and the incidence and mortality rates are increasing at an alarming rate. The aim of the present study was to investigate the effect of swimming training and royal jelly consumption at different doses on IL-6 and LPS-induced IKB-Z gene expression in mice with benzo[a]pyrene -induced lung cancer. Material & Methods: 48 male Balb/c mice were randomly assigned to 8 groups: healthy control, sham, benzopyrene, swimming training, royal jelly 50, royal jelly 100, swimming training + royal jelly 100, swimming training + royal jelly 50. Lung cancer was induced by injecting 100 mg/kg of benzopyrene. Swimming training was performed three times a week for 12 weeks. Royal jelly was also injected intraperitoneally at doses of 50 and 100 mg/kg of body weight daily for 12 weeks. 48 hours after the last training session, LPS-Induced IKB-Z and IL-6 gene expression was measured using Real-Time PCR. Results: The expression of LPS-induced IKB-Z and IL-6 genes was significantly increased in the benzopyrene group compared to the healthy control group (p=0.001). The expression of these two genes was lower in the royal jelly 100 and royal jelly + swimming training groups compared to the benzopyrene group (p=0.001). In addition, the expression of LPS-induced IKB-Z and IL-6 was lower in the swimming training group compared to the benzopyrene group (p=0.004 and p=0.001, respectively). Also, the expression of IL-6 gene was lower in the royal jelly 50 group compared to the benzopyrene group (p=0.011). Conclusion: It seems that although exercise and royal jelly supplementation, especially at higher doses, can be effective in reducing inflammation in lung tissue, the combined effect of the two, especially at higher doses, can have a greater modulating effect against inflammation and lung cancer in conditions of exposure to benzopyrene.