This protocol enables the investigation of electrical properties of hPSC-derived 3D cardiac organoids at both single-cell and tissue levels using MEA and sharp electrode techniques.
Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.
Venkateshappa et al. (Thu,) studied this question.