What question did this study set out to answer?

To determine whether novel frameshift peptides generated by microsatellite instability in mismatch repair-deficient tumors can stimulate tumor-specific cytotoxic T-cell responses.

January 1, 2001International Journal of CancerOpen Access

Frameshift peptide-derived T-cell epitopes: A source of novel tumor-specific antigens

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Authors

MLMichael LinnebacherUniversity of Rostock JGJohannes GebertHeidelberg University WRW. RudyMicro Materials (United Kingdom)

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Implication

Experimental study demonstrates cytotoxic T-cell targeting of frameshift peptides in colorectal cancer, suggesting viable avenues for therapeutic and preventive cancer vaccines.

Key Points

To determine whether novel frameshift peptides generated by microsatellite instability in mismatch repair-deficient tumors can stimulate tumor-specific cytotoxic T-cell responses.
Generated cytotoxic T lymphocytes (CTLs) by stimulating cells with peptide-pulsed autologous CD40-activated B cells against HLA-A2.1-restricted frameshift sequences.
Screened 16 candidate frameshift peptides derived from recurrent mutations across 8 genes in microsatellite-unstable (MSI) tumors.
Tested CTL-mediated lysis against target cells loaded with synthetic peptides and the MSI colorectal cancer cell line HCT116 carrying an endogenous TGFbetaIIR mutation.
Identified 3 out of 16 predicted frameshift peptides that mediated specific cytotoxic T lymphocyte lysis of peptide-loaded target cells.
Demonstrated that a CTL bulk culture directed against a (-1) frameshift mutation in TGFbetaIIR successfully lysed the mutant HCT116 colorectal cancer cell line.

Cite This Study

Linnebacher et al. (2001) studied this question.

synapsesocial.com/papers/69ff4cf6581c6e761e77635a https://doi.org/10.1002/ijc.1298

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Implication

Experimental study demonstrates cytotoxic T-cell targeting of frameshift peptides in colorectal cancer, suggesting viable avenues for therapeutic and preventive cancer vaccines.

Frameshift peptide-derived T-cell epitopes: A source of novel tumor-specific antigens

Discussion

Member takes

Implication

Key Points

Cite This Study

Frameshift peptide-derived T-cell epitopes: A source of novel tumor-specific antigens

Discussion

Member takes

Implication

Key Points

Cite This Study

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