What question did this study set out to answer?

To isolate, fractionate, and structurally characterize the primary polysaccharides present within the fruiting bodies of cultivated Pleurotus ostreatus.

July 28, 2019MoleculesOpen Access

Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

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Authors

EBEkaterina BaevaUniversity of Chemistry and Technology, Prague RBRoman BlehaUniversity of Chemistry and Technology, Prague ELEkaterina LavrovaUniversity of Chemistry and Technology, Prague

Discussion

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Implication

Biochemical analysis reveals diverse glucan and mannogalactan fractions in Pleurotus ostreatus basidiocarps, highlighting complex fungal cell wall architecture.

Key Points

To isolate, fractionate, and structurally characterize the primary polysaccharides present within the fruiting bodies of cultivated Pleurotus ostreatus.
Extracted basidiocarp polysaccharides sequentially using cold water, hot water, and two consecutive treatments with 1 M sodium hydroxide.
Separated polysaccharide components using two-stage chromatography and dimethyl sulfoxide (DMSO) fractionation.
Cold-water and hot-water extractions predominantly yielded branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan, respectively.
Fractionation of the primary alkaline extract using DMSO isolated insoluble linear (1→3)-α-d-glucan and soluble branched (1→3)(1→6)-β-d-glucans.
Secondary alkaline extraction released branched β-d-glucans bound to proteins, leaving an insoluble cell wall residue composed of a chitin and β-d-glucan complex.

Cite This Study

Baeva et al. (2019) studied this question.

synapsesocial.com/papers/69ff97ae2ff633f365779b1b https://doi.org/10.3390/molecules24152740

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July 28, 2019MoleculesOpen Access

Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

View Full Paper

Ask AI

Bookmark

Authors

EBEkaterina BaevaUniversity of Chemistry and Technology, Prague RBRoman BlehaUniversity of Chemistry and Technology, Prague ELEkaterina LavrovaUniversity of Chemistry and Technology, Prague

Discussion

Member takes

Implication

Biochemical analysis reveals diverse glucan and mannogalactan fractions in Pleurotus ostreatus basidiocarps, highlighting complex fungal cell wall architecture.

Key Points

To isolate, fractionate, and structurally characterize the primary polysaccharides present within the fruiting bodies of cultivated Pleurotus ostreatus.
Extracted basidiocarp polysaccharides sequentially using cold water, hot water, and two consecutive treatments with 1 M sodium hydroxide.
Separated polysaccharide components using two-stage chromatography and dimethyl sulfoxide (DMSO) fractionation.
Cold-water and hot-water extractions predominantly yielded branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan, respectively.
Fractionation of the primary alkaline extract using DMSO isolated insoluble linear (1→3)-α-d-glucan and soluble branched (1→3)(1→6)-β-d-glucans.
Secondary alkaline extraction released branched β-d-glucans bound to proteins, leaving an insoluble cell wall residue composed of a chitin and β-d-glucan complex.

Cite This Study

Baeva et al. (2019) studied this question.

synapsesocial.com/papers/69ff97ae2ff633f365779b1b https://doi.org/10.3390/molecules24152740

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