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This research aims to evaluate the effectiveness of Bna.SCT and Bna.REF1 as target genes for reducing sinapine in rapeseed.

May 10, 2026Open Access

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Evaluation of Bna.SCT and Bna.REF1 as Target Genes to Reduce Sinapine in Rapeseed Using a Protoplast‐Based CRISPR RNP Approach

OMOliver MossSwedish University of Agricultural Sciences XLXueyuan LiSwedish University of Agricultural Sciences SKSelvaraju KanagarajanSwedish University of Agricultural Sciences

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This research aims to evaluate the effectiveness of Bna.SCT and Bna.REF1 as target genes for reducing sinapine in rapeseed.
Utilized a CRISPR RNP approach to manipulate gene targets.
Assessed sinapine content in rapeseed protoplasts with single sgRNA for both genes.
Monitored plant growth and development post-manipulation.
Sinapine content was reduced by up to 38% in Bna.SCT mutants.
Sinapine content was reduced by 77% in Bna.REF1 mutants.
No negative effects on plant growth or development were observed.

Resumen

plants with a single sgRNA. Seed sinapine content was reduced by up to 38% in Bna.SCT mutants and 77% in Bna.REF1 mutants, with no observed effects on plant growth or development. These findings suggest that Bna.REF1 is the most effective target for sinapine reduction in transgene-free mutants among the genes tested in our lab.

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Evaluation of Bna.SCT and Bna.REF1 as Target Genes to Reduce Sinapine in Rapeseed Using a Protoplast‐Based CRISPR RNP Approach

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