ABSTRACT Aim Species Distribution Models (SDMs) are widely used in conservation planning, invasive species management and global change assessments. Their reliability depends on both presence and absence data, yet biodiversity databases are dominated by presence records, while true absences are rarely collected and geographically restricted. We introduce a framework that integrates large language models (LLMs) to sample ecologically realistic pseudo‐absences under the constraints of dendritic river networks. Innovation We present the Language‐Grounded Multivariate Pseudo‐Absence Sampling (LGMPAS) framework, which converts environmental predictor profiles into natural‐language eco‐narratives that an LLM uses to score and rank pre‐filtered candidate locations drawn exclusively from the river network and restricted to unlabelled sites. Retrieval‐Augmented Generation (RAG) further anchors eco‐narratives in published ecological knowledge. We tested LGMPAS on two ecologically contrasting crayfish species in the Danube basin, the widespread invasive Faxonius limosus and the narrowly endemic Austropotamobius bihariensis , validating outputs against independent field‐collected true‐absence data using Random Forest predictive performance, spatial overlap of high‐suitability areas and predictor‐space distances. LLM‐derived pseudo‐absences closely reproduced true‐absence model outputs and consistently outperformed random sampling across both species. Main Conclusions LGMPAS demonstrates that LLMs can reliably sample pseudo‐absences that reproduce the ecological signal of true‐absence data, even under complex freshwater network constraints. By reducing dependence on costly absence surveys in contexts where true‐absence data are unavailable or spatially restricted, and by avoiding the biases inherent to random sampling, the framework strengthens the robustness of SDMs for conservation applications. Its reproducibility and adaptability across taxa and ecosystems offer particular value for biodiversity monitoring, invasive species management and conservation planning under global change.
Miok et al. (Fri,) studied this question.