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December 14, 2022Frontiers in OncologyOpen Access

Plasma exosomal miR-320d, miR-4479, and miR-6763-5p as diagnostic biomarkers in epithelial ovarian cancer

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Authors

SWShiwen WangSun Yat-sen UniversityXSXingguo SongShandong First Medical UniversityXSXingguo SongShandong First Medical University

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Implication

Biomarker study reveals downregulation of three plasma exosomal microRNAs in epithelial ovarian cancer, indicating their potential as noninvasive diagnostic markers.

Key Points

  • To validate the diagnostic accuracy of plasma exosomal miR-320d, miR-4479, and miR-6763-5p as circulating biomarkers for epithelial ovarian cancer.
  • Isolated plasma exosomes using ultracentrifugation and validated them using transmission electron microscopy, qNano, and western blotting.
  • Identified candidate microRNAs via sequencing and validated them with RT-qPCR in 161 primary epithelial ovarian cancer patients and 168 healthy donors, alongside benign controls.
  • Assessed the diagnostic accuracy of the microRNAs using receiver operating characteristic curve analysis.
  • Identified 95 differentially expressed exosomal microRNAs, with miR-320d, miR-4479, and miR-6763-5p significantly downregulated in ovarian cancer patients compared to healthy and benign controls.
  • Demonstrated diagnostic biomarker capacity with areas under the curve of 0.6549 for miR-320d, 0.7781 for miR-4479, and 0.6834 for miR-6763-5p.
  • Observed significant correlations between all three exosomal microRNAs and lymph node metastasis, with miR-320d and miR-4479 also associating with tumor stage.

Cite This Study

Wang et al. (2022) studied this question.

synapsesocial.com/papers/6a006ac064548b97a42d84e0https://doi.org/10.3389/fonc.2022.986343
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