What question did this study set out to answer?

To develop a sensitive ELISA inhibition assay utilizing the monoclonal antibody M86 for precise quantification of alpha-gal epitope expression on cells and tissues.

April 1, 1998Transplantation

A Sensitive Assay for Measuring ??-Gal Epitope Expression on Cells by a Monoclonal Anti-Gal Antibody1

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Authors

UGUri GaliliRush University Medical Center DLDenise C. LaTempleAllegheny College MRMarko RadicUniversity of Tennessee Health Science Center

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Implication

Assay development study demonstrates sensitive quantification of alpha-gal epitopes on cells and tissues, highlighting utility for evaluating genetically modified xenografts.

Key Points

To develop a sensitive ELISA inhibition assay utilizing the monoclonal antibody M86 for precise quantification of alpha-gal epitope expression on cells and tissues.
Incubated various cell concentrations overnight with a 1/100 dilution of the mouse IgM monoclonal anti-Gal antibody M86.
Pelleted cells to collect supernatant containing unbound antibody and quantified residual M86 using an ELISA with alpha-gal-bovine serum albumin as the solid-phase antigen.
Generated inhibition binding curves against standard cell lines with known epitope counts to evaluate cell suspensions and tissue homogenate membranes.
The M86 monoclonal IgM antibody exhibited high specificity for the alpha-gal epitope (Galalpha1-3Galbeta1-4GlcNAc-R).
The ELISA inhibition assay accurately detected alpha-gal expression across a dynamic range of 5 x 10^4 to more than 5 x 10^7 epitopes per cell.
The method successfully quantified decreases in alpha-gal epitope expression on membranes derived from genetically manipulated animal tissue homogenates.

Cite This Study

Galili et al. (1998) studied this question.

synapsesocial.com/papers/6a01ec75897643a80dcb1d06 https://doi.org/10.1097/00007890-199804270-00020

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Implication

Assay development study demonstrates sensitive quantification of alpha-gal epitopes on cells and tissues, highlighting utility for evaluating genetically modified xenografts.

A Sensitive Assay for Measuring ??-Gal Epitope Expression on Cells by a Monoclonal Anti-Gal Antibody1

Discussion

Member takes

Implication

Key Points

Cite This Study

A Sensitive Assay for Measuring ??-Gal Epitope Expression on Cells by a Monoclonal Anti-Gal Antibody1

Discussion

Member takes

Implication

Key Points

Cite This Study

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